Range of biochips

Design of a biochip-based test system
Design of a biochip-based test system.

The diagnosis process with the help of a biochip is based on the principle of direct fluorescent immunocytochemistry with subsequent morphological analysis.

A standard biochip has compartments covered with a protective film. These compartments contain fluorescent-labelled proteins.

We presently offer 7 types of test systems:

 

Range of biochips:
ЕРН – for determining the immunophenotype of breast cancer cells;
SER – for determining the nature of the serous fluid;
Tyr – for differential diagnosis of thyroid neoplasms;
Pulmo – for diagnosing lung cancer;
PNV – for diagnosing malignant tumours in the genitourinary tract;
BigTarget – to search for markers for drug treatment;
NOD1 и NOD2 – a wide panel;

 

A biochip is a plate whose dimensions are 25 mm х 75 mm and which is 1 mm thick. The surface of a bioship is divided into two sections: one section contains a transparent plate made of glass which has a positively charged coating layer and forms the working surface of the biochip; the other surface has no specific coating, is intended for holding the biochip by hands and contains the information on cell localization. Test compartments contain protein ligands conjugated with a fluorescent label, for example, Su3. The working surface of the biochip is covered with an airtight and sterile film.

Biochips are stored in a lightproof container before their use. The time of their exposure to light may not exceed 20 minutes before the reaction. It is required to protect the product from direct sunlight when it has been taken out of the container.

Biochips make it clinically possible:
To make histogenetic diagnosis of tumours
To define the nosological variant of a growth
To detect the primary tumour by metastasis with an unknown primary site
To make a prognosis of tumour development
To recognise a malignant transformation of cells
To find grounds for prescribing targeted therapy
To analyse both the resistance or sensitivity

of tumour cells to chemotherapeutic and target compounds

ЕРН BIOCHIP

Designed for differential diagnosis of breast cancer and target search for drug treatment.

The test system has 3 lines of 5 markers:

List of markers
Estrogen receptor (ER)
Progesterone receptors (РGR)
Her2-neu (EGFR 2)
EGFR
Ki67

The development helps optimise the tumour antigenic status analysis process in case of breast cancer while reducing the time and cost of the study and preserving the same information contents.

SER BIOCHIP

Resolved problem: determination of the nature of the serous fluid with source verification (in case of malignant genesis).

List of markers
СА-125
РЭА
Cytokeratin 7 (СК7)
Cytokeratin 20 (СК 20)
РЭ (ER)
TTF
WT-1
BerEp4
Vimentin (VIM)

Test materials: serous fluid.

TYR BIOCHIP:

Purpose: differential diagnosis of thyroid neoplasms.

List of markers
Thyroglobulin (TG)
Calcitonin (CT)
TTF1
Galectin 3 (GAL 3)
Mesothelial antigen НВМЕ1
Cytokeratin 19 (СК19)
CD44v6 (HCAM)
p53

Test materials: FNAB.

Pulmo BIOCHIP

Purpose: differential diagnosis of lung neoplasms.

List of markers
СК7
СК 20
TTF1
Chromogranin А
Cinaptofizin
p53
CD 56
СК5/6
р63

Test materials: FNAB, bronchial washing material, tumour fragments.

PNV BIOCHIP

Purpose: differential diagnosis of bladder neoplasms, bladder cancer screening.

List of markers
р16 (CDKN2A)
Ki67
р63
Cytokeratins 5/6 (СК5/6)
CK7
CK 20
СА125
WT1
Uroplakin (UPK3А)
FAS (CD95)
CDX2

Materials are: urine, bladder washout materials.

NOD1 BIOCHIP

Purpose: determination which organ the cells belong to.

List of markers
РЭ (ER)
СК7
СК20
ПСА (PSA)
Calretinin (СR)
Chromogranin А
CD56 (NCAM)
CD10 (NEP)
CD44 (HCAM)
CK19
RCC
TTF1
ЭМА
Thyroglobulin (TG)
CDX2

Test materials: FNAB, tumour fragments.

NOD2 BIOCHIP

Purpose: determination which organ the cells belong to.

List of markers
СК7
СК20
CD138 (SDC1)
Vimentin (VIM)
ЕрСam
CD99
S100
Desmin
ЭМА
Melan А (MLANA)
НМВ 45 (ME20M)
РЭА
HBME1
Osteonectin (ОN)
Osteocalcin (OC)

Test materials: FNAB, tumour fragments.